| 88 | Sam Aiken | 28 | wr | 14 | 2 | 204 | 6-2 | North Carolina | 12/14/1980 | 5 | 1 | Buffalo Bills / 4th / 127th pick / 2003 |
| 52 | Eric Alexander | 26 | | 1 | 0 | 230 | 6-3 | LSU | 2/8/1982 | 4 | 0 | |
| 12 | Tom Brady | 31 | qb | 1 | 1 | 225 | 6-4 | Michigan | 8/3/1977 | 8 | 0 | New England Patriots / 6th / 199th pick / 2000 |
| 65 | Wesley Britt | 27 | | 2 | 0 | 314 | 6-8 | Alabama | 11/21/1981 | 2 | 0 | San Diego Chargers / 5th / 164th pick / 2005 |
| 54 | Tedy Bruschi | 35 | RILB | 13 | 12 | 247 | 6-1 | Arizona | 6/9/1973 | 12 | 6 | New England Patriots / 3rd / 86th pick / 1996 |
| 16 | Matt Cassel | 26 | QB | 16 | 15 | 230 | 6-5 | USC | 5/17/1982 | 3 | 17 | New England Patriots / 7th / 230th pick / 2005 |
| 95 | Rosevelt Colvin | 31 | lb | 4 | 3 | 250 | 6-3 | Purdue | 9/5/1977 | 9 | 1 | Chicago Bears / 4th / 111th pick / 1999 |
| 63 | Daniel Connolly | 26 | | 1 | 0 | 311 | 6-4 | SE Missouri St. | 9/2/1982 | 3 | 0 | |
| 85 | Tyson DeVree | 24 | | 2 | 0 | 245 | 6-6 | Colorado | 11/12/1984 | Rook | 0 | |
| 44 | Heath Evans | 30 | fb | 16 | 4 | 245 | 6-0 | Auburn | 12/30/1978 | 7 | 3 | Seattle Seahawks / 3rd / 82nd pick / 2001 |
| 33 | Kevin Faulk | 32 | rb | 15 | 3 | 202 | 5-8 | LSU | 6/5/1976 | 9 | 10 | New England Patriots / 2nd / 46th pick / 1999 |
| 10 | Jabar Gaffney | 28 | wr | 16 | 7 | 205 | 6-1 | Florida | 12/1/1980 | 6 | 5 | Houston Texans / 2nd / 33rd pick / 2002 |
| 3 | Stephen Gostkowski*+ | 24 | K | 16 | 0 | 200 | 6-2 | Memphis | 1/28/1984 | 2 | 0 | New England Patriots / 4th / 118th pick / 2006 |
| 97 | Jarvis Green | 29 | de | 14 | 3 | 290 | 6-3 | LSU | 1/12/1979 | 6 | 3 | New England Patriots / 4th / 126th pick / 2002 |
| 42 | BenJarvus Green-Ellis | 23 | rb | 9 | 3 | 215 | 5-11 | Mississippi | 7/2/1985 | Rook | 3 | |
| 59 | Gary Guyton | 23 | lb | 15 | 2 | 245 | 6-2 | Georgia Tech | 11/14/1985 | Rook | 3 | |
| 6 | Chris Hanson | 32 | P | 16 | 0 | 223 | 6-2 | Marshall | 10/25/1976 | 9 | 0 | |
| 37 | Rodney Harrison | 36 | ss | 6 | 6 | 220 | 6-1 | West. Illinois | 12/15/1972 | 14 | 3 | San Diego Chargers / 5th / 145th pick / 1994 |
| 27 | Ellis Hobbs | 25 | KR | 16 | 16 | 188 | 5-9 | Iowa St. | 5/16/1983 | 3 | 9 | New England Patriots / 3rd / 84th pick / 2005 |
| 71 | Russ Hochstein | 31 | c | 15 | 2 | 305 | 6-4 | Nebraska | 10/7/1977 | 6 | 2 | Tampa Bay Buccaneers / 5th / 151st pick / 2001 |
| 53 | Larry Izzo | 34 | | 16 | 0 | 228 | 5-10 | Rice | 9/26/1974 | 12 | 1 | |
| 32 | LaMont Jordan | 30 | | 8 | 0 | 230 | 5-10 | Maryland | 11/11/1978 | 7 | 3 | New York Jets / 2nd / 49th pick / 2001 |
| 77 | Nick Kaczur | 29 | RT | 14 | 14 | 319 | 6-4 | Toledo | 7/28/1979 | 3 | 9 | New England Patriots / 3rd / 100th pick / 2005 |
| 67 | Dan Koppen | 29 | C | 16 | 16 | 296 | 6-2 | Boston Col. | 9/12/1979 | 5 | 8 | New England Patriots / 5th / 164th pick / 2003 |
| 64 | Mark LeVoir | 26 | t | 16 | 2 | 306 | 6-7 | Notre Dame | 7/29/1982 | Rook | 2 | |
| 72 | Matt Light | 30 | LT | 16 | 16 | 305 | 6-4 | Purdue | 6/23/1978 | 7 | 10 | New England Patriots / 2nd / 48th pick / 2001 |
| 70 | Logan Mankins | 26 | LG | 16 | 16 | 307 | 6-4 | Fresno St. | 3/10/1982 | 3 | 8 | New England Patriots / 1st / 32nd pick / 2005 |
| 39 | Laurence Maroney | 23 | rb | 3 | 3 | 210 | 5-11 | Minnesota | 2/5/1985 | 2 | 1 | New England Patriots / 1st / 21st pick / 2006 |
| 51 | Jerod Mayo | 22 | LILB | 16 | 16 | 230 | 6-2 | Tennessee | 2/23/1986 | Rook | 9 | New England Patriots / 1st / 10th pick / 2008 |
| 31 | Brandon Meriweather | 24 | SS | 16 | 11 | 195 | 5-11 | Miami (FL) | 1/14/1984 | 1 | 6 | New England Patriots / 1st / 24th pick / 2007 |
| 34 | Sammy Morris | 31 | RB | 13 | 7 | 220 | 6-0 | Texas Tech | 3/23/1977 | 8 | 8 | Buffalo Bills / 5th / 156th pick / 2000 |
| 81 | Randy Moss | 31 | WR | 16 | 16 | 215 | 6-4 | Marshall | 2/13/1977 | 10 | 11 | Minnesota Vikings / 1st / 21st pick / 1998 |
| 61 | Steve Neal | 32 | RG | 11 | 9 | 305 | 6-4 | Cal State-Bakersfield | 10/9/1976 | 6 | 5 | |
| 5 | Kevin O'Connell | 23 | | 2 | 0 | 225 | 6-5 | San Diego St. | 5/25/1985 | Rook | 0 | New England Patriots / 3rd / 94th pick / 2008 |
| 21 | Deltha O'Neal | 31 | LCB | 16 | 10 | 194 | 5-11 | California | 1/30/1977 | 8 | 5 | Denver Broncos / 1st / 15th pick / 2000 |
| 66 | Lonie Paxton | 30 | | 16 | 0 | 260 | 6-2 | Sacramento St. | 3/13/1978 | 8 | 1 | |
| 49 | Vince Redd | 23 | | 5 | 0 | 260 | 6-6 | Liberty | 9/1/1985 | Rook | 0 | |
| 35 | Mike Richardson | 24 | | 10 | 0 | 190 | 5-11 | Notre Dame | 2/18/1984 | Rook | 1 | New England Patriots / 6th / 202nd pick / 2007 |
| 36 | James Sanders | 25 | FS | 14 | 14 | 207 | 5-10 | Fresno St. | 11/11/1983 | 3 | 6 | New England Patriots / 4th / 133rd pick / 2005 |
| 29 | Lewis Sanders | 30 | db | 10 | 4 | 210 | 6-1 | Maryland | 6/22/1978 | 8 | 2 | Cleveland Browns / 4th / 95th pick / 2000 |
| 55 | Junior Seau | 39 | lb | 4 | 2 | 250 | 6-3 | USC | 1/19/1969 | 18 | 1 | San Diego Chargers / 1st / 5th pick / 1990 |
| 93 | Richard Seymour | 29 | RDE | 15 | 15 | 310 | 6-6 | Georgia | 10/6/1979 | 7 | 8 | New England Patriots / 1st / 6th pick / 2001 |
| 18 | Matt Slater | 23 | | 14 | 0 | 198 | 6-0 | UCLA | 9/9/1985 | Rook | 0 | New England Patriots / 5th / 153rd pick / 2008 |
| 90 | LeKevin Smith | 26 | | 15 | 0 | 310 | 6-1 | Nebraska | 7/21/1982 | 2 | 2 | New England Patriots / 6th / 206th pick / 2006 |
| 82 | Stephen Spach | 26 | | 2 | 0 | 250 | 6-4 | Fresno St. | 7/18/1982 | 3 | 0 | |
| 28 | Antwain Spann | 25 | | 10 | 0 | 190 | 6-0 | La-Lafayette | 2/22/1983 | 2 | 1 | |
| 96 | Adalius Thomas | 31 | ROLB | 9 | 9 | 270 | 6-2 | Southern Miss | 8/17/1977 | 8 | 4 | Baltimore Ravens / 6th / 186th pick / 2000 |
| 86 | David Thomas | 25 | TE | 15 | 10 | 240 | 6-3 | Texas | 7/5/1983 | 2 | 3 | New England Patriots / 3rd / 86th pick / 2006 |
| 41 | Raymond Ventrone | 22 | | 15 | 0 | 200 | 5-10 | Villanova | 10/22/1986 | 1 | 1 | |
| 50 | Mike Vrabel | 33 | LOLB | 16 | 14 | 261 | 6-4 | Ohio St. | 8/14/1975 | 11 | 7 | Pittsburgh Steelers / 3rd / 91st pick / 1997 |
| 94 | Ty Warren | 27 | LDE | 13 | 13 | 300 | 6-5 | Texas A&M | 2/6/1981 | 5 | 7 | New England Patriots / 1st / 13th pick / 2003 |
| 15 | Kelley Washington | 29 | | 10 | 0 | 218 | 6-3 | Tennessee | 8/21/1979 | 5 | 0 | Cincinnati Bengals / 3rd / 65th pick / 2003 |
| 84 | Ben Watson | 28 | TE | 14 | 9 | 255 | 6-3 | Duke,Georgia | 12/18/1980 | 4 | 4 | New England Patriots / 1st / 32nd pick / 2004 |
| 23 | Jason Webster | 31 | | 3 | 0 | 187 | 5-9 | Texas A&M | 9/8/1977 | 8 | 0 | San Francisco 49ers / 2nd / 48th pick / 2000 |
| 83 | Wes Welker* | 27 | PR | 16 | 14 | 190 | 5-9 | Texas Tech | 5/1/1981 | 4 | 13 | |
| 22 | Terrence Wheatley | 23 | db | 6 | 1 | 187 | 5-10 | Colorado | 5/5/1985 | Rook | 1 | New England Patriots / 2nd / 62nd pick / 2008 |
| 75 | Vince Wilfork | 27 | NT | 16 | 16 | 325 | 6-2 | Miami (FL) | 11/4/1981 | 4 | 8 | New England Patriots / 1st / 21st pick / 2004 |
| 24 | Jonathan Wilhite | 24 | db | 16 | 4 | 183 | 5-9 | Auburn | 2/23/1984 | Rook | 3 | New England Patriots / 4th / 129th pick / 2008 |
| 58 | Pierre Woods | 26 | lb | 12 | 3 | 249 | 6-5 | Michigan | 1/6/1982 | 2 | 2 | |
| 99 | Mike Wright | 26 | dt | 16 | 2 | 295 | 6-4 | Ashland,Cincinnati | 3/1/1982 | 3 | 3 | |
| 74 | Billy Yates | 28 | rg | 7 | 7 | 305 | 6-2 | Texas A&M | 4/15/1980 | 5 | 4 | |