| 92 | Cliff Avril | 25 | DE | 16 | 16 | 252 | 6-3 | Purdue | 4/8/1986 | 3 | 9 | Detroit Lions / 3rd / 92nd pick / 2008 |
| 76 | Jeff Backus | 34 | LT | 16 | 16 | 305 | 6-5 | Michigan | 9/21/1977 | 10 | 9 | Detroit Lions / 1st / 18th pick / 2001 |
| 32 | Aaron Berry | 23 | cb | 11 | 3 | 180 | 5-11 | Pittsburgh | 6/25/1988 | 1 | 2 | |
| 44 | Jahvid Best | 22 | rb | 6 | 6 | 190 | 5-9 | California | 1/30/1989 | 1 | 5 | Detroit Lions / 1st / 30th pick / 2010 |
| 21 | Aaron Brown | 26 | | 1 | 0 | 196 | 6-0 | TCU | 10/10/1985 | 2 | 0 | Detroit Lions / 6th / 192nd pick / 2009 |
| 35 | Eldra Buckley | 26 | | 2 | 0 | 202 | 5-9 | Tenn-Chattanooga | 6/23/1985 | 2 | 0 | |
| 13 | Nate Burleson | 30 | WR | 16 | 11 | 192 | 6-0 | Nevada-Reno | 8/19/1981 | 8 | 7 | Minnesota Vikings / 3rd / 71st pick / 2003 |
| 31 | Don Carey | 24 | | 5 | 0 | 186 | 5-11 | Norfolk State | 2/14/1987 | 1 | 0 | Cleveland Browns / 6th / 177th pick / 2009 |
| 59 | Bobby Carpenter | 28 | lb | 16 | 3 | 255 | 6-3 | Ohio St. | 8/1/1983 | 5 | 3 | Dallas Cowboys / 1st / 18th pick / 2006 |
| 77 | Gosder Cherilus | 27 | RT | 16 | 15 | 315 | 6-7 | Boston Col. | 6/28/1984 | 3 | 9 | Detroit Lions / 1st / 17th pick / 2008 |
| 24 | Erik Coleman | 29 | | 4 | 0 | 200 | 5-10 | Washington St. | 5/6/1982 | 7 | 0 | New York Jets / 5th / 143rd pick / 2004 |
| 81 | Rashied Davis | 32 | | 11 | 0 | 180 | 5-10 | San Jose St. | 7/24/1979 | 6 | 1 | |
| 62 | Keyunta Dawson | 26 | | 2 | 0 | 268 | 6-0 | Texas Tech | 9/13/1985 | 4 | 0 | Indianapolis Colts / 7th / 242nd pick / 2007 |
| 26 | Louis Delmas | 24 | S | 11 | 11 | 197 | 5-11 | West. Michigan | 4/12/1987 | 2 | 5 | Detroit Lions / 2nd / 33rd pick / 2009 |
| 6 | Ryan Donahue | 23 | | 8 | 0 | 190 | 6-3 | | 3/17/1988 | Rook | 0 | |
| 52 | Justin Durant | 26 | LB | 13 | 12 | 230 | 6-4 | Hampton | 9/21/1985 | 4 | 6 | Jacksonville Jaguars / 2nd / 48th pick / 2007 |
| 50 | Isaiah Ekejiuba | 30 | | 3 | 0 | 219 | 6-4 | Virginia | 10/5/1981 | 6 | 0 | |
| 98 | Nick Fairley | 23 | | 10 | 0 | 291 | 6-4 | Auburn | 1/23/1988 | Rook | 1 | Detroit Lions / 1st / 13th pick / 2011 |
| 96 | Andre Fluellen | 26 | | 13 | 0 | 285 | 6-2 | Florida St. | 3/7/1985 | 3 | 1 | Detroit Lions / 3rd / 87th pick / 2008 |
| 38 | Vincent Fuller | 29 | | 1 | 0 | 189 | 6-1 | Virginia Tech | 8/3/1982 | 6 | 0 | Tennessee Titans / 4th / 108th pick / 2005 |
| 65 | Dylan Gandy | 29 | | 16 | 0 | 300 | 6-3 | Texas Tech | 3/8/1982 | 6 | 1 | Indianapolis Colts / 4th / 129th pick / 2005 |
| 2 | Ben Graham | 38 | | 7 | 0 | 230 | 6-5 | Deakin (Australia) | 11/2/1973 | 6 | 0 | |
| 4 | Jason Hanson | 41 | | 16 | 0 | 182 | 5-11 | Washington St. | 6/17/1970 | 19 | 0 | Detroit Lions / 2nd / 56th pick / 1992 |
| 43 | Chris Harris | 29 | s | 8 | 5 | 206 | 6-1 | La-Monroe | 8/6/1982 | 6 | 2 | Chicago Bears / 6th / 181st pick / 2005 |
| 36 | Jerome Harrison | 28 | | 4 | 0 | 195 | 5-9 | Washington St. | 2/26/1983 | 5 | 0 | Cleveland Browns / 5th / 145th pick / 2006 |
| 95 | Jovan Haye | 29 | | 1 | 0 | 284 | 6-2 | Vanderbilt | 6/21/1982 | 6 | 0 | Carolina Panthers / 6th / 189th pick / 2005 |
| 89 | Will Heller | 30 | te | 16 | 7 | 250 | 6-6 | Georgia Tech | 2/28/1981 | 8 | 2 | |
| 91 | Sammie Lee Hill | 25 | dt | 16 | 3 | 331 | 6-4 | Stillman | 11/8/1986 | 2 | 3 | Detroit Lions / 4th / 115th pick / 2009 |
| 14 | Shaun Hill | 31 | | 2 | 0 | 210 | 6-5 | Maryland | 1/9/1980 | 6 | 0 | |
| 78 | Corey Hilliard | 26 | rt | 16 | 1 | 315 | 6-5 | Oklahoma St. | 4/26/1985 | 4 | 2 | New England Patriots / 6th / 209th pick / 2007 |
| 52 | Doug Hogue | 22 | | 13 | 0 | 235 | 6-2 | Syracuse | 2/1/1989 | Rook | 1 | Detroit Lions / 5th / 157th pick / 2011 |
| 23 | Chris Houston | 27 | CB | 14 | 13 | 185 | 5-10 | Arkansas | 10/18/1984 | 4 | 7 | Atlanta Falcons / 2nd / 41st pick / 2007 |
| 94 | Lawrence Jackson | 26 | | 11 | 0 | 261 | 6-5 | USC | 8/30/1985 | 3 | 2 | Seattle Seahawks / 1st / 28th pick / 2008 |
| 81 | Calvin Johnson*+ | 26 | WR | 16 | 16 | 239 | 6-5 | Georgia Tech | 9/25/1985 | 4 | 15 | Detroit Lions / 1st / 2nd pick / 2007 |
| 54 | DeAndre Levy | 24 | LB | 16 | 16 | 230 | 6-2 | Wisconsin | 3/26/1987 | 2 | 8 | Detroit Lions / 3rd / 76th pick / 2009 |
| 11 | Stefan Logan | 30 | wr | 16 | 1 | 177 | 5-7 | South Dakota | 6/2/1981 | 2 | 0 | |
| 30 | Anthony Madison | 30 | | 5 | 0 | 180 | 5-9 | Alabama | 10/8/1981 | 5 | 0 | |
| 2 | Robert Malone | 23 | | 1 | 0 | 215 | 6-2 | Fresno St. | 2/4/1988 | 1 | 0 | |
| 33 | Brandon McDonald | 26 | | 13 | 0 | 181 | 5-11 | Memphis | 8/26/1985 | 4 | 1 | Cleveland Browns / 5th / 140th pick / 2007 |
| 28 | Maurice Morris | 32 | rb | 16 | 5 | 202 | 5-11 | Oregon | 12/1/1979 | 9 | 4 | Seattle Seahawks / 2nd / 54th pick / 2002 |
| 48 | Don Muhlbach | 30 | | 16 | 0 | 262 | 6-5 | Texas A&M | 8/17/1981 | 7 | 0 | |
| 58 | Ashlee Palmer | 25 | | 16 | 0 | 223 | 6-1 | Mississippi | 4/7/1986 | 2 | 1 | |
| 66 | Stephen Peterman | 29 | RG | 16 | 16 | 318 | 6-4 | LSU | 1/11/1982 | 6 | 8 | Dallas Cowboys / 3rd / 83rd pick / 2004 |
| 84 | Brandon Pettigrew | 26 | TE | 16 | 16 | 257 | 6-5 | Oklahoma St. | 2/23/1985 | 2 | 9 | Detroit Lions / 1st / 20th pick / 2009 |
| 51 | Dominic Raiola | 33 | C | 16 | 16 | 295 | 6-1 | Nebraska | 12/30/1978 | 10 | 8 | Detroit Lions / 2nd / 50th pick / 2001 |
| 85 | Tony Scheffler | 28 | te | 15 | 5 | 255 | 6-5 | West. Michigan | 2/15/1983 | 5 | 5 | Denver Broncos / 2nd / 61st pick / 2006 |
| 39 | Ricardo Silva | 23 | | 4 | 0 | 225 | 6-3 | Hampton | 5/9/1988 | Rook | 0 | |
| 67 | Rob Sims | 28 | G | 16 | 16 | 320 | 6-3 | Ohio St. | 12/6/1983 | 5 | 8 | Seattle Seahawks / 4th / 128th pick / 2006 |
| 27 | Alphonso Smith | 26 | cb | 11 | 1 | 193 | 5-9 | Wake Forest | 10/20/1985 | 2 | 2 | Denver Broncos / 2nd / 37th pick / 2009 |
| 30 | Kevin Smith | 25 | rb | 7 | 4 | 210 | 6-0 | Central Florida | 12/17/1986 | 3 | 4 | Detroit Lions / 3rd / 64th pick / 2008 |
| 42 | Amari Spievey | 23 | S | 15 | 15 | 190 | 6-0 | Iowa | 4/14/1988 | 1 | 7 | Detroit Lions / 3rd / 66th pick / 2010 |
| 9 | Matthew Stafford | 23 | QB | 16 | 16 | 230 | 6-2 | Georgia | 2/7/1988 | 2 | 16 | Detroit Lions / 1st / 1st pick / 2009 |
| 80 | Maurice Stovall | 26 | | 15 | 0 | 215 | 6-4 | Notre Dame | 2/21/1985 | 5 | 0 | Tampa Bay Buccaneers / 3rd / 90th pick / 2006 |
| 90 | Ndamukong Suh | 24 | DT | 14 | 14 | 307 | 6-4 | Nebraska | 1/6/1987 | 1 | 6 | Detroit Lions / 1st / 2nd pick / 2010 |
| 55 | Stephen Tulloch | 26 | LB | 16 | 16 | 230 | 5-11 | North Carolina St. | 1/1/1985 | 5 | 9 | Tennessee Titans / 4th / 116th pick / 2006 |
| 93 | Kyle Vanden Bosch | 33 | DE | 16 | 16 | 278 | 6-4 | Nebraska | 11/17/1978 | 10 | 8 | Arizona Cardinals / 2nd / 34th pick / 2001 |
| 29 | John Wendling | 28 | s | 16 | 1 | 222 | 6-1 | Wyoming | 6/4/1983 | 4 | 1 | Buffalo Bills / 6th / 184th pick / 2007 |
| 99 | Corey Williams | 31 | DT | 15 | 15 | 313 | 6-4 | Arkansas St. | 8/17/1980 | 7 | 7 | Green Bay Packers / 6th / 179th pick / 2004 |
| 34 | Keiland Williams | 25 | | 15 | 0 | 225 | 5-11 | LSU | 8/14/1986 | 1 | 2 | |
| 21 | Eric Wright | 26 | DB | 16 | 16 | 192 | 5-11 | UNLV | 7/24/1985 | 4 | 8 | Cleveland Browns / 2nd / 53rd pick / 2007 |
| 16 | Titus Young | 22 | WR | 16 | 9 | 174 | 5-11 | Boise State | 8/21/1989 | Rook | 5 | Detroit Lions / 2nd / 44th pick / 2011 |