| 29 | Joseph Addai | 23 | | 16 | 0 | 210 | 5-11 | LSU | 5/3/1983 | Rook | 15 | Indianapolis Colts / 1st / 30th pick / 2006 |
| 41 | Antoine Bethea | 22 | SS | 14 | 14 | 203 | 5-11 | Howard | 7/27/1984 | Rook | 5 | Indianapolis Colts / 6th / 207th pick / 2006 |
| 50 | Rocky Boiman | 26 | lb | 16 | 1 | 236 | 6-4 | Notre Dame | 1/24/1980 | 4 | 1 | Tennessee Titans / 4th / 133rd pick / 2002 |
| 58 | Gary Brackett | 26 | MLB | 14 | 14 | 235 | 5-11 | Rutgers | 5/23/1980 | 3 | 5 | |
| 79 | Raheem Brock | 28 | RDT | 16 | 16 | 274 | 6-4 | Temple | 6/10/1978 | 4 | 6 | Philadelphia Eagles / 7th / 238th pick / 2002 |
| 35 | Ran Carthon | 25 | | 3 | 0 | 218 | 6-0 | Florida | 2/10/1981 | 1 | 0 | |
| 44 | Dallas Clark | 27 | TE | 12 | 12 | 257 | 6-3 | Iowa | 6/12/1979 | 3 | 7 | Indianapolis Colts / 1st / 24th pick / 2003 |
| 42 | Jason David | 24 | RCB | 16 | 16 | 165 | 5-8 | Washington St. | 6/12/1982 | 2 | 6 | Indianapolis Colts / 4th / 125th pick / 2004 |
| 71 | Ryan Diem | 27 | RT | 15 | 15 | 331 | 6-6 | Northern Illinois | 7/1/1979 | 5 | 10 | Indianapolis Colts / 4th / 118th pick / 2001 |
| 30 | De De Dorsey | 22 | | 13 | 0 | 193 | 6-0 | Lindenwood | 8/1/1984 | Rook | 0 | |
| 20 | Mike Doss | 25 | db | 6 | 4 | 207 | 5-10 | Ohio St. | 3/24/1981 | 3 | 2 | Indianapolis Colts / 2nd / 58th pick / 2003 |
| 76 | Daniel Federkeil | 23 | | 2 | 0 | 290 | 6-6 | Calgary | 11/9/1983 | Rook | 0 | |
| 81 | Bryan Fletcher | 27 | te | 15 | 3 | 238 | 6-5 | UCLA | 3/23/1979 | 1 | 4 | Chicago Bears / 6th / 210th pick / 2002 |
| 93 | Dwight Freeney | 26 | RDE | 16 | 16 | 268 | 6-1 | Syracuse | 2/19/1980 | 4 | 5 | Indianapolis Colts / 1st / 11th pick / 2002 |
| 57 | Dylan Gandy | 24 | LG | 16 | 11 | 300 | 6-3 | Texas Tech | 3/8/1982 | 1 | 6 | Indianapolis Colts / 4th / 129th pick / 2005 |
| 51 | Gilbert Gardner | 24 | LLB | 15 | 12 | 228 | 6-1 | Purdue | 5/9/1982 | 2 | 4 | Indianapolis Colts / 3rd / 69th pick / 2004 |
| 43 | Matt Giordano | 24 | db | 12 | 1 | 194 | 5-11 | California | 10/16/1982 | 1 | 1 | Indianapolis Colts / 4th / 135th pick / 2005 |
| 78 | Tarik Glenn* | 30 | LT | 16 | 16 | 332 | 6-5 | California | 5/25/1976 | 9 | 14 | Indianapolis Colts / 1st / 19th pick / 1997 |
| 7 | Martin Gramatica | 31 | | 3 | 0 | 170 | 5-8 | Kansas St. | 11/27/1975 | 7 | 0 | Tampa Bay Buccaneers / 3rd / 80th pick / 1999 |
| 56 | Tyjuan Hagler | 25 | | 9 | 0 | 236 | 6-0 | Cincinnati | 12/3/1981 | Rook | 0 | Indianapolis Colts / 5th / 173rd pick / 2005 |
| 25 | Nick Harper | 32 | LCB | 15 | 15 | 182 | 5-10 | Fort Valley State (GA) | 9/10/1974 | 5 | 6 | |
| 88 | Marvin Harrison*+ | 34 | WR | 16 | 16 | 175 | 6-0 | Syracuse | 8/25/1972 | 10 | 17 | Indianapolis Colts / 1st / 19th pick / 1996 |
| 26 | Kelvin Hayden | 23 | db | 15 | 1 | 198 | 5-10 | Illinois | 7/23/1983 | 1 | 2 | Indianapolis Colts / 2nd / 60th pick / 2005 |
| 28 | Marlin Jackson | 23 | FS | 14 | 8 | 196 | 6-0 | Michigan | 6/30/1983 | 1 | 3 | Indianapolis Colts / 1st / 29th pick / 2005 |
| 27 | Tim Jennings | 23 | | 11 | 0 | 186 | 5-8 | Georgia | 12/24/1983 | Rook | 1 | Indianapolis Colts / 2nd / 62nd pick / 2006 |
| 74 | Charlie Johnson | 22 | t | 16 | 1 | 302 | 6-3 | Oklahoma St. | 5/2/1984 | Rook | 2 | Indianapolis Colts / 6th / 199th pick / 2006 |
| 59 | Cato June | 27 | RLB | 16 | 16 | 227 | 6-0 | Michigan | 11/18/1979 | 3 | 7 | Indianapolis Colts / 6th / 198th pick / 2003 |
| 54 | Freddie Keiaho | 24 | | 14 | 0 | 224 | 5-11 | San Diego St. | 12/18/1982 | Rook | 1 | Indianapolis Colts / 3rd / 94th pick / 2006 |
| 92 | Dan Klecko | 25 | | 10 | 0 | 275 | 5-11 | Temple | 1/12/1981 | 3 | 1 | New England Patriots / 4th / 117th pick / 2003 |
| 99 | Ryan LaCasse | 23 | | 12 | 0 | 257 | 6-2 | Syracuse | 2/6/1983 | Rook | 1 | Baltimore Ravens / 7th / 219th pick / 2006 |
| 65 | Ryan Lilja | 25 | lg | 11 | 5 | 285 | 6-2 | Kansas St. | 10/15/1981 | 2 | 3 | |
| 18 | Peyton Manning* | 30 | QB | 16 | 16 | 230 | 6-5 | Tennessee | 3/24/1976 | 8 | 20 | Indianapolis Colts / 1st / 1st pick / 1998 |
| 98 | Robert Mathis | 25 | LDE | 16 | 16 | 235 | 6-2 | Alabama A&M | 2/26/1981 | 3 | 7 | Indianapolis Colts / 5th / 138th pick / 2003 |
| 92 | Anthony McFarland | 29 | LDT | 11 | 11 | 300 | 6-0 | LSU | 12/18/1977 | 7 | 4 | Tampa Bay Buccaneers / 1st / 15th pick / 1999 |
| 85 | Aaron Moorehead | 26 | | 12 | 0 | 200 | 6-3 | Illinois | 11/5/1980 | 3 | 1 | |
| 94 | Rob Morris | 31 | llb | 15 | 5 | 243 | 6-2 | BYU | 1/18/1975 | 6 | 2 | Indianapolis Colts / 1st / 28th pick / 2000 |
| 53 | Keith O'Neil | 26 | | 10 | 0 | 230 | 6-0 | Northern Arizona | 8/26/1980 | 3 | 1 | |
| 11 | Ricky Proehl | 38 | wr | 2 | 1 | 190 | 6-0 | Wake Forest | 3/7/1968 | 16 | 0 | Phoenix Cardinals / 3rd / 58th pick / 1990 |
| 90 | Montae Reagor | 29 | ldt | 5 | 5 | 285 | 6-3 | Texas Tech | 6/29/1977 | 7 | 2 | Denver Broncos / 2nd / 58th pick / 1999 |
| 95 | Darrell Reid | 24 | | 15 | 0 | 288 | 6-2 | Minnesota | 6/20/1982 | 1 | 1 | |
| 36 | Dexter Reid | 25 | db | 10 | 1 | 200 | 5-11 | North Carolina | 3/18/1981 | 2 | 1 | New England Patriots / 4th / 113th pick / 2004 |
| 33 | Dominic Rhodes | 27 | RB | 16 | 16 | 203 | 5-9 | Midwestern St. | 1/17/1979 | 5 | 9 | |
| 34 | T.J. Rushing | 23 | | 6 | 0 | 175 | 5-10 | Stanford | 6/8/1983 | Rook | 0 | Indianapolis Colts / 7th / 238th pick / 2006 |
| 21 | Bob Sanders | 25 | db | 4 | 4 | 200 | 5-8 | Iowa | 2/24/1981 | 2 | 2 | Indianapolis Colts / 2nd / 44th pick / 2004 |
| 63 | Jeff Saturday* | 31 | C | 16 | 16 | 292 | 6-2 | North Carolina | 6/8/1975 | 7 | 14 | |
| 64 | Bo Schobel | 25 | | 14 | 0 | 268 | 6-5 | TCU | 3/24/1981 | 2 | 1 | Tennessee Titans / 4th / 103rd pick / 2004 |
| 73 | Jake Scott | 25 | RG | 16 | 16 | 283 | 6-5 | Idaho | 4/16/1981 | 2 | 9 | Indianapolis Colts / 5th / 141st pick / 2004 |
| 17 | Hunter Smith | 29 | P | 16 | 0 | 209 | 6-2 | Notre Dame | 8/9/1977 | 7 | 0 | Indianapolis Colts / 7th / 210th pick / 1999 |
| 48 | Justin Snow | 30 | | 16 | 0 | 240 | 6-3 | Baylor | 12/21/1976 | 6 | 1 | |
| 12 | Jim Sorgi | 26 | | 1 | 0 | 196 | 6-5 | Wisconsin | 12/3/1980 | 2 | 0 | Indianapolis Colts / 6th / 193rd pick / 2004 |
| 83 | Brandon Stokley | 30 | wr | 4 | 1 | 197 | 5-11 | La-Lafayette | 6/23/1976 | 7 | 1 | Baltimore Ravens / 4th / 105th pick / 1999 |
| 91 | Josh Thomas | 25 | | 14 | 0 | 271 | 6-5 | Syracuse | 6/26/1981 | 2 | 1 | |
| 69 | Matt Ulrich | 25 | | 5 | 0 | 309 | 6-2 | Northwestern | 12/30/1981 | 1 | 0 | |
| 86 | Ben Utecht | 25 | TE | 15 | 15 | 251 | 6-6 | Minnesota | 6/30/1981 | 1 | 8 | |
| 4 | Adam Vinatieri | 34 | K | 13 | 0 | 202 | 6-0 | South Dakota St. | 12/28/1972 | 10 | 0 | |
| 87 | Reggie Wayne* | 28 | WR | 16 | 16 | 198 | 6-0 | Miami (FL) | 11/17/1978 | 5 | 17 | Indianapolis Colts / 1st / 30th pick / 2001 |
| 10 | Terrence Wilkins | 31 | KR | 15 | 0 | 180 | 5-10 | Virginia | 7/29/1975 | 7 | 1 | |